bioREACTOR - High-throughput bioprocess development in the milliliter scale

Designed for the scale-down of biotechnological production processes into the milliliter-scale, developed for efficient process development and high throughput screenings in the biotechnological, chemical and pharmaceutical research, simple handling with very high time and cost savings due to the parallelization and miniaturization.

bioREACTOR 48

The 2mag bioREACTOR is a new developed, space-saving and user-friendly bioreaction block with up to 48 parallelized mini reactors.

BIOREAKTOR-- 012
The high parallelization allows an easy realization of high-throughput fermentations in the biotechnological, chemical or pharmaceutical research. This strongly reduces development time and costs for new production processes. Miniaturization into the low milliliter scale (8-15 ml) provides a significant saving of material and process costs.





The 2mag bioREACTOR is characterized by a precise temperature control, an exactly controllable stirring speed with automatic stirrer monitoring as well as precise non-invasive real-time measurement of pH and dissolved oxygen (DO).
Gassing and mixing of the reaction vessels is provided by the gas inducing, inductive stirring elements.



BIOREAKTOR-BEAUTY-- 015 150 The sterile headspace aeration prevents cross and external contaminations and allows cultivation of aerobic and anaerobic microorganisms. An easy and secure scaling-up of the results into production scale can be ensured by precisely defined engineering parameters and the comparable power and oxygen input (kLa > 0,4 s-1) to well-established stirred tank reactors.
The bioREACTOR can be operated as standalone or fully automated by integration into a pipetting robot.



Advantages

wirbel mini High parallelization   wirbel mini Automated stirrer monitoring
wirbel mini Precise temperature control   wirbel mini Intelligent software for parallel operation
wirbel mini Optimal working volume   wirbel mini Autoclavability and high sterility
wirbel mini Non-invasive real-time measurement of pH and DO   wirbel mini Easy Upscaling
wirbel mini Easy handling through disposables   wirbel mini Automatization
wirbel mini High oxygen transfer rates   wirbel mini High parallel and sequential reproducibility



Type bioREACTOR 48
Stirring points 48
Stirring point distance 35 mm
Material block Aluminium, hard-coated
Dimensions (WxDxH) 240 x 340 x 145 mm
Weight (gross) approx. 16 kg
Electrical data 230 V / 100 W
Protection category IP31
Reaction vessels
Working volume 8 - 15 ml
Material Polystyrene (PS)
Temperature
Operating conditions +10 °C up to +65 °C (at 80% humidity)
Mixing
Stirring element gas inducing stirring elements
Stirring speed 100 - 4,000 rpm
Gassing
Gassing rate 4.8 l/min
Real-time measurement
pH range 6.0 bis 8.5
DO range 0 - 50 % O2
Response time (pH/DO) < 30 s (at 15 - 45°C)
Order No B70048


  2 years warranty on material and manufacturing.

We reserve the right to make technical alterations. We do not assume liability for errors in printing or typeing.


Parallelization
The 2mag bioREACTOR is available in two model variants with 8 or 48 parallel reaction vessels. A still higher parallelization can be achieved by simoultaneous use of several bioREACTOR units. These can be controlled via an intelligent central software and important process parameters (pH ans DO) can be monitored in real-time. In addition, the simoultaneous use of several bioREACTOR units allows the variation of other parameters e.g. the temperature or the stirring speed.

Temperature Control
The temperature of the bioreactor block is recognized by an internal  Pt100 sensor and controlled externally by a thermostat. To avoid evaporation loss, the headspace of the reaction vessels is cooled down with an reflux cooler. The evaporated water condenses and rests in the reaction vessel.

Working Volume
The working volume of the reaction vessels ranges between 8 and 15 milliliters. This allows repeated sampling without strong reduction of the fermentation volume but also enough capacity for adding pH correction agents or substrates.

Real-time measurement of pH and DO
pH and dissolved oxygen can be measured in real-time with a non-invasive optical sensor system over the whole process time. For this, the bottom of the reaction vessels are equipped with small sensor spots (pH and O2 sensitive luminescent dyes). The fluorometric measurement is provided by stimulation with light and measurement of the decay time.  (DLR=Dual Lifetime Referencing Principle, PreSens GmbH). The measuring range lies between 6.0 and 8.5 for the pH and between 0 and 50 % for the dissolved oxygen.

Reaction vessels (Disposables)
The spotted disposable reaction vessels are made of polystyrene and sterile packed in a set of two. This allows a time saving and easy handling of the bioREACTOR. The special geometry of the reaction vessels with baffles in combination with the used stirring elements lead to a very good mixing of the medium and to high oxygen transfer rates.  

Gassing
The continuous gassing of the bioREACTOR can be realized with a gas mixing station. Depending on the used gases (O2, N2, CO2, Air,…) aerobic and anaerobic cultivations are possible. The gas transfer into the medium of the reaction vessels is done passively through the gas inducing, inductive driven magnetic stirring elements. The gas tranfer rate can be adjusted via the stirring speed.

Stirrer monitoring
At very high cell densities or a high percentage of solids in the medium a stop of rare stirrers can occur in less cases. This will be detected in a few seconds and the stirrers will be restarted automatically. Therewith, the stirrer monitoring guarantees a secure experimental run.

Intelligent software
The stand alone software allows the parallel use of several bioREACTOR 8 and/or 48 units. At the same time, already finished runs can be compared with new running experiments.

Autoclavability and sterility
All parts which can be in contact with the medium are autoclavable or sterile packed disposables. The gassing unit with the fixed stirring elements is completely autoclavable. In addition, the complete bioreactor block is sterilizable with ethanol. The disposable reaction vessels are sterile packed and were disposed after the experiment. During the experiment, the sterile headspace gassing avoids cross and external contaminations.  Furthermore, an optional exhaust gas unit can avoid spreeding of spores into the environment.

Scalability
The exactly defined geometry of the stirring elements, the precisely characterized process parameters as well as the to standard laboratory stirred tank reactors comparable power and oxygen input (kLa > 0,4 s-1) through the stirring elements allow an easy upscaling of the results into the laboratory and production scale.

Automatization
The bioREACTOR can be used as stand alone or fully automated by integration into a pipetting robot. The integration allows pH control, adding of substrates and taking samples out of the reaction vessels. Therewith feeding strategies can be analyzed, an online measurement of the optical density (OD) can be made or samples for additional tests can be taken out of the vessels..



Please contact us for more information.


wirbel mini     bioREACTOR 48
wirbel mini     bioREACTORcontrol 48
wirbel mini     Accessories (maintenance tool, steriBOX)
wirbel mini     PreSens sensor system for non-invasive pH- and DO-measurement
wirbel mini     Stand-Alone Software

Peripheral units e.g. thermostats, reflux cooler or gas mixing stations on request!

wirbel mini     Aerobic and anaerobic cultivation of bacteria, yeasts and funghi
wirbel mini     Evaluation of adequate production strains (strain development)
wirbel mini     Media screening and optimization
wirbel mini     Optimization of the process design (manual batch or automated batch or fed-batch process design with pipetting robots)
wirbel mini     Cell growth experiments
wirbel mini     Studies of gene and protein expression
wirbel mini     Viabily tests
wirbel mini     Inhibition and toxicity experiments
wirbel mini     Enzyme assays
wirbel mini     Quality control
wirbel mini     Also applicable for chemical and enzymatic reactions

wirbel mini     Production of Vitamin B2 with Bacillus subtilis [1]
wirbel mini     Enzymatic hydrolysis of plant materials [2]
wirbel mini     Production of bio butanol with Clostridium acetobutylicum [3]
wirbel mini     Production of a antimycotic with Streptomyces tendae [4]
wirbel mini     Production of acetic acid from synthetic gas with Acetobacterium woodii [5]
wirbel mini     Feeding strategies of Saccharomyces cerevsiae [6]
wirbel mini     Production of hydroxyisobuturic acid with Cupriavidus necator [7]
wirbel mini     Fluxome analysis of Escherichia coli mutants [8]

Literatur
[1]     Vester A, Hans M, Hohmann P, Weuster-Botz D (2009): Discrimination of riboflavin producing Bacillus subtilis strains based on their fed-batch process performances on a millilitre scale. Appl Microbiol Biotechnol 84: 71-76
[2]     Riedlberger P, Weuster-Botz D (2012): New miniature stirred-tank bioreactors for parallel study of enzymatic biomass hydrolysis. Bioresource Technol 106: 138-146
[3]    Schmidt M, Weuster-Botz D (2012): Reaction engineering studies of acetone-butanol-ethanol fermentation with Clostridium acetobutylicum. Biotechnol J 7: 656-661
[4]    Hortsch R, Krispin H, Weuster-Botz D (2010): Process performance of parallel bioreactors for batch cultivation of Streptomyces tendae. Bioproc Biosys Eng, 34: 297-304
[5]     Demler M, Weuster-Botz D (2011): Reaction engineering analysis of hydrogenotrophic production of acetic acid by Acetobacterium woodii. Biotechnol Bioeng 108: 470-474
[6]    Gebhardt G, Hortsch R, Kaufmann K, Arnold M, Weuster-Botz D (2011): A new microfluidic concept for parallel operated milliliter-scale stirred tank bioreactors. Biotechnol Prog Vol. 27, No. 3.
[7]    Höfel T, Wittmann E, Reinecke L, Weuster-Botz D (2010): Reaction engineering studies for the production of 2-hydroxyisobutyric acid with recombinant Cupriavidus necator H16. Appl Microbiol Biotechnol 88: 477-484.
[8]    Heux S, Poinot J, Massou S, Sokol S, Portais JC (2014): A novel platform for automated high-throughput fluxome profiling of metabolic variants. Metabolic Engineering 25:8-19.

Type bioREACTOR 48 bioREACTOR 8
Stirring points 48 8
Stirring point distance 35 mm
Material block Aluminium, hard-coated
Dimensions (WxDxH) 240 x 340 x 145 mm 66 x 340 x 145 mm
Weight (gross) approx. 16 kg approx. 3.7 kg
Electrical data 230 V / 1000 W 230 V / 50 W
Protection category IP31
Reaction vessels
Working volume 8 - 15 ml
Material Polystyrene (PS)
Temperature
Operating conditions +10 °C up to +65 °C (at 80% humidity)
Mixing
Stirring element gas inducing stirring elements
Stirring speed 100 - 4,000 rpm
Gassing
Gassing rate 4.8 l/min 0.8 l/min
Real-time measurement
pH range 6.0 bis 8.5
DO range 0 - 50 % O2
Response time (pH/DO) < 30 s (at 15 - 45°C)
Order No B70048 B70008


  2 years warranty on material and manufacturing.

We reserve the right to make technical alterations. We do not assume liability for errors in printing or typeing.


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